Shanghai Zhichu Instrument Co., Ltd.
Shanghai Zhichu Instrument Co., Ltd.

A biological safety cabinet is a critical containment device designed for safe handling of hazardous materials (pathogens, toxins) while protecting operators, samples, and the environment. Available in Class I, II (A2/B2), and III, it features HEPA filtration (99.97% @ 0.3µm), laminar airflow, and UV sterilization. Essential for microbiology, virology, and pharmaceutical labs, it ensures compliance with biosafety levels (BSL-2/3/4).

What Does a Biological Safety Cabinet Do?

What Does a Biological Safety Cabinet Do?

A Biological Safety Cabinet (BSC) is a ventilated enclosure designed to provide personnel, environmental, and product protection during work with hazardous biological materials.

Primary Functions:

  • Personnel Protection

  • HEPA-filtered exhaust air prevents exposure to pathogens (e.g., viruses, bacteria).

  • Product Protection

  • Sterile airflow (ISO Class 5) safeguards samples from contamination.

  • Environmental Protection


Filters exhaust air (HEPA/ULPA) before release into the lab or outside.

Types of BSCs:

  • Class I: Protects user and environment only (no product protection).

  • Class II (Most Common):

    Type A2: Recirculates 70% air, exhausts 30% (for low-moderate risk agents).

    Type B2: 100% exhaust (for toxic chemicals/volatiles).

  • Class III: Fully sealed, glove-box style (for high-risk pathogens like Ebola).


How to Work in a Biological Safety Cabinet?

  • Turn on early
    Run the cabinet 10–15 minutes before starting to stabilize airflow.

  • Disinfect surfaces
    Clean all interior surfaces with 70% alcohol before and after use.

  • Arrange items properly
    Separate clean and dirty areas; do not block air grilles.

  • Work slowly
    Use smooth, slow movements to avoid disturbing the airflow.

  • Wear PPE
    Always wear gloves, lab coat, and eye protection.

  • Clean after work
    Disinfect all surfaces again and let the cabinet run before shutting down.

How to Work in a Biological Safety Cabinet?
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